Papillary thyroid cancer (PTC) is the most common type of cancer among thyroid malignancies. Tumor‐related methylation of circulating tumor DNA (ctDNA) in plasma could represent tumor specific alterations can be considered as good biomarkers in circulating tumor cells. In this study, we studied the methylation status of seven promoter regions of two DNA methyl Transferases (MGMT and DNMT1) genes as the methylated ctDNA in plasma and tissue samples of patients with PTC and goiter patients as noncancerous controls.
Methods: Both ctDNA and tissue genomic DNA of 57 PTC and 45 Goiter samples were isolated. After bisulfite modification, the methylation status was studied by Methylation‐Sensitive High Resolution Melting (MS‐HRM) assay technique. Four promoter regions of O6‐methylguanine‐DNA methyltransferase (MGMT) and three promoter regions of DNA methyltransferase 1 (DNMT1) were …
F Khatami, L Teimoori‐Toolabi, R Heshmat, S Nasiri… - Journal of Cellular Biochemistry, 2019
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