Wnt signaling is hyper‐activated in most of human cancers including colorectal carcinoma (CRC). Therefore, the introduction of new regulators for Wnt pathway possesses promising diagnostic and therapeutic applications in cancer medicine. Bioinformatics analysis introduced hsa‐miR‐103a, hsa‐miR‐1827, and hsa‐miR‐137 as potential regulators of Wnt signaling pathway. Here, we intended to examine the effect of these human miRNAs on Wnt signaling pathway components, on the cell cycle progression in CRC originated cell lines and their expression in CRC tissues. RT‐qPCR results indicated upregulation of hsa‐miR‐103a, hsa‐miR‐1827, and downregulation of hsa‐miR‐137 in CRC tissues. Overexpression of hsa‐miR‐103a and hsa‐miR‐1827 in SW480 cells resulted in elevated Wnt activity, detected by both Top/Flash assay and RT‐qPCR analysis. Inhibition of Wnt signaling by using PNU‐74654 or …

Introduction of hsa‐miR‐103a and hsa‐miR‐1827 and hsa‐miR‐137 as new regulators of Wnt signaling pathway and their relation to colorectal carcinoma

A Fasihi, B M. Soltani, A Atashi, S Nasiri - Journal of cellular biochemistry, 2018