Methods:

In this research, we determined the promoter methylation status of four tumor suppressor genes (SLC5A8, RASSF1, MGMT, and DNMT1) and compared the results of 55 PTC cases with 40 goiter patients. For methylation, we used the methylation-sensitive high resolution melting (MS-HRM) assay technique. The resulting graphs of each run were compared with those of 0%, 50%, and 100% methylated controls.

Results:

Our data showed that the promoter methylation of SLC5A8, Ras association domain family member 1 (RASSF1), and MGMT were significantly different between PTC tissue and goiter with P-value less than 0.05. The most significant differences were observed in RASSF1; 77.2% of hyper-methylated PTC patients versus 15.6% hyper-methylated goiter samples (P< 0.001).

Conclusion:

RASSF1 promoter methylation can be a PTC genetic marker. RASSF1 promoter methylation is under the …

Promoter methylation of four tumor suppressor genes in human papillary thyroid carcinoma

F Khatami, B Larijani, R Heshmat, S Nasiri, H Saffar… - Iranian Journal of Pathology, 2019